ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the efficiency of the BIOMED-2 PCR assay and its implication in the diagnosis of mature B-cell non-Hodgkin's lymphomas.</p><p><b>METHODS</b>Clinical, morphological and immunohistochemical features of 72 cases of non-Hodgkin's lymphomas were studied, including 25 reactive lymphoid hyperplasia, 37 diffuse large B cell lymphomas (DLBCL) and 35 extranodal marginal zone lymphomas of mucosa associated lymphoid tissues (MALT lymphoma and in addition, 25 cases of reactive lymphoid hyperplasia were used as the controls). DNA was exacted from the paraffin embedded formalin fixed tissue blocks and the quality of DNA was assessed using the BIOMED-2 specimen control reaction. Adequate samples were then analyzed by BIOMED-2 for immunoglobulin heavy and kappa light chain rearrangements.</p><p><b>RESULTS</b>Adequate DNA was obtained in 83 of 97 samples, including 60 mature B cell lymphomas and 23 reactive lymphoid hyperplasia. Clonal B-cell gene rearrangements were detected in 57 of 60 (95%) lymphomas. In contrast, clonal Ig gene rearrangements were not detected in any of the 23 cases of reactive lymphoid hyperplasia.</p><p><b>CONCLUSION</b>BIOMED-2 assay is highly sensitive and specific for the detection of clonal B cell gene rearrangement using routine paraffin embedded formalin fixed specimens.</p>
Subject(s)
Humans , Antigens, CD20 , Metabolism , CD79 Antigens , Metabolism , DNA, Neoplasm , Genetics , Gene Rearrangement, B-Lymphocyte , Genetics , Gene Rearrangement, B-Lymphocyte, Heavy Chain , Genetics , Gene Rearrangement, B-Lymphocyte, Light Chain , Genetics , Genes, Immunoglobulin , Immunophenotyping , Lymphoma, B-Cell , Genetics , Allergy and Immunology , Pathology , Lymphoma, B-Cell, Marginal Zone , Genetics , Allergy and Immunology , Pathology , Lymphoma, Large B-Cell, Diffuse , Genetics , Allergy and Immunology , Pathology , Paraffin Embedding , Pseudolymphoma , Genetics , Allergy and Immunology , Pathology , Sensitivity and SpecificityABSTRACT
<p><b>OBJECTIVE</b>To biodegrade the diesel pollution in aqueous solution inoculated with Mycobacterium and filamentous fungi.</p><p><b>METHODS</b>Bacteria sampled from petroleum hydrocarbons contaminated sites in Karamay Oilfield were isolated and identified as Mycobacterium hyalinum (MH) and cladosporium. Spectrophotometry and gas chromatography (GC) were used to analyze of the residual concentrations of diesel oil and its biodegradation products.</p><p><b>RESULTS</b>From the GC data, the values of apparent biodegradation ratio of the bacterial strain MH to diesel oil were close to those obtained in the control experiments. Moreover, the number of MH did not increase with degradation time. However, by using n-octadecane instead of diesel oil, the real biotic degradation ratio increased to 20.9% over 5 days of degradation. Cladosporium strongly biodegraded diesel oil with a real degradation ratio of up to 34% after 5 days treatment. When the two strains were used simultaneously, a significant synergistic effect between them resulted in almost complete degradation of diesel oil, achieving a total diesel removal of 99% over 5 days of treatment, in which one part of about 80% and another part of about 19% were attributed to biotic and abiotic processes, respectively.</p><p><b>CONCLUSION</b>The observed synergistic effect was closely related to the aromatics-degrading ability of Cladosporium, which favored the growth of MH and promoted the bioavailability of diesel oil.</p>